Mouse THB ELISA Kit

Mouse THB ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to THB, During the reaction,THB in the sample or standard competes with a fixed amount of biotin-labeled THB for sites on a pre-coated Monoclonal antibody specific to THB. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of THB in the samples is then determined by comparing the O.D. of the samples to the standard curve. Species : Mouse Storage: Store the whole kit at 4℃ Samples: Gene: THB Uniprot AC: Intended Use: Mouse THB ELISA Kitallows for the in vitro quantitative determination of THB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.