Mouse TTG-lgA ELISA Kit

Intended Uses: This TTGAb ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse TTGAb. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay:TTGAb ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TTGAb antibody and an TTGAb-HRP conjugate. The assay sample and buffer are incubated together with TTGAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TTGAb concentration since TTGAb from samples and TTGAb-HRP conjugate compete for the anti-TTGAb antibody binding site. Since the number of sites is limited, as more sites are occupied by TTGAb from the sample, fewer sites are left to bind TTGAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TTGAb concentration in each sample is interpolated from this standard curve. Species : Mouse Storage: For 5-7days:Store the whole kit at 4℃For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃. Samples: Serum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids. Gene: TTG-lgA Uniprot AC: Intended Use: Mouse TTG-lgA ELISA Kitallows for the in vitro quantitative determination of TTG-lgA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.